Oncogenomics Shared Resource Facility (OG SRF)

OG SRF Overview

The overall mission of the Oncogenomics Shared Resource Facility (OG SRF) laboratory is to accelerate the clinical genomics, precision medicine and genomic research interests at the Markey Cancer Center (MCC) by providing cutting-edge genomic and molecular technology services.

Functioning as one component of the larger UK HealthCare Genomics Core, the OG SRF provides state-of-the-art technologies specifically adapted to needs of MCC investigators to facilitate genomics into the translational pathway from basic research to clinical and population-based studies. The OG SRF plays a critical role in Markey’s directions for research and delivery of precision medicine. 

The Genomics Core Laboratory, a College of American Pathologists-accredited facility, was established in 2015 as part of UK HealthCare, Department of Pathology and Laboratory Medicine and MCC's Genomics initiative. The facility occupies about 4,500 square feet within the UK HealthCare Clinical Laboratories. 

You can browse our pages here to learn about our team, find our location and other vital information. For more, visit the Genomics Core Laboratory webpage.

Specific Aims

  1. Provide comprehensive and high-quality genomic services.
  2. Provide genomic consultation services.
  3. Enhance coordinated operations with project relevant SRFs to support the team science research model of MCC.

We provide investigators access to a wide range of genomic services, including the following:

Investigation Platform Consultation

The expert team of OG SRF works with investigators on choosing appropriate research methods and platforms to fit their scientific and budgetary needs.

It is strongly recommended to schedule a consultation with OG SRF to help you select the best methods for your project.  Selections are based on considerations such as cutting-edge platforms available, cost efficiency, high-quality deliverables and providing basic genomic data interpretation. 

OG SRF works closely with the Biostatistics and Bioinformatics SRF to analyze and interpret your data. Click here to see Bioinformatics Services.

Next-Generation Sequencing

The Oncogenomics Shared Resource Facility offers library preparation and Illumina sequencing services. Library preparation can be performed on the Agilent Bravo, and sequencing services are available on the MiSeq, NextSeq 2000, and NovaSeq 6000.

DNA Sequencing

  • Library Preparation Services
  • Whole genome sequencing
  • Targeted sequencing
    • Amplicon sequencing and small gene panels
    • Investigators provide the probes/amplicons/libraries
  • Whole exome sequencing
    • Agilent Clinical Research Exome
    • Agilent Focused Exome
    • Agilent All Human Exon

RNA Sequencing

  • mRNA Seq for differential expression experiments
  • Whole transcriptome sequencing (RNA-seq)

Single-Cell Sequencing

  • 10x Genomics Chromium

Sanger Sequencing

  • Tier I – Sequencing only
    • Investigators perform PCR, dye incorporation, and dye cleanup
  • Tier II – Clean up and sequencing 
    • Investigator perform PCR and dye incorporation
  • Tier III – Sanger sequencing reaction, cleanup and sequencing
    • Investigator perform PCR

Fragment Analysis

  • Investigator submits amplicons for fragment analysis on the ABI 3730

Gene Expression Microarray

  • All standard arrays
  • miRNA array
  • Custom arrays

Misc. Services

  • NanoString Sprint
  • Digital PCR
  • Nucleic acid QC (Bioanalyzer, TapeStation, Qubit)
  • Covaris ultrasonication

Please complete your service-specific request form and submit to genomics@uky.edu.

Visit the Genomics Core Laboratory webpage for more information.

Shulin Zhang

Shulin Zhang, MD, PhD
OG SRF Director

sainan wei

Sainan Wei, MD, PhD
OG SRF Associate Director

ryan vincent

Ryan Vincent, BS, MB(ASCP)
OG Laboratory Manager

 

UK HealthCare Genomics Core Laboratory
UK Albert B. Chandler Hospital - Pavilion HA
Room HA629
800 Rose St.
Lexington KY 40536-0293
Call 859-323-5327
Call 859-323-5984
Email genomics@uky.edu

Note that this email is not secure and should not be used to send personal health information.
 

Visit the Genomics Core Laboratory website for more information and additional pricing information.

quotation marks

Acknowledge the OG SRF

Investigators are required to acknowledge the Markey Cancer Center Oncogenomics Shared Resource Facility (OG SRF) in any publications that result from the use of services or information received through the MCC OG SRF. Please use the following statement to acknowledge the OG SRF.

“This research was supported by the Oncogenomics Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558).”

Markey Cancer Center is NCI-designated

The UK Markey Cancer Center was first designated by the National Cancer Institute in 2013 – a distinction that recognizes our extraordinary ability to provide world-class care for our patients. We are the only NCI-designated cancer center in Kentucky and one of only 71 in the nation.

LEARN MORE