Flow Cytometry and Immune Monitoring Shared Resource Facility

FCIM SRF Overview

The overall mission of the Flow Cytometry and Immune Monitoring Shared Resource Facility (FCIM SRF) is to provide technical and scientific support for MCC investigators whose research programs require flow cytometric cell analysis and/or sorting or other immune-based cell assays. FCIM provides access to a team of expert immunologists and state of art instruments to aid pre-clinical, translational, and clinical research activities at the Markey Cancer Center. The FCIM team comprises experts in flow cytometry, tumor immunology, immunotherapy, vaccine biology, inflammation, autoimmune diseases, immune biomarker discovery, drug development and testing. We provide both fee-based services and scientific collaboration for research requiring immune system expertise for both human or animal studies.

Visit the full Flow Cytometry and Immune Monitoring Shared Resource Facility site for more information.

Full FCIM Website

Specific Aims

  1. Provide cost-effective state-of-the-art flow cytometry and cell sorting services for basic, preclinical and clinical researchers.
  2. Provide cost-effective state-of-the-art immune monitoring services for basic, pre-clinical and clinical researchers.
  3. Provide educational training and mentoring to MCC members and/or laboratory staff in proper use of self-service instrumentation and appropriate experimental design and data analysis.
  • Flow cytometric analysis 
    • Cell phenotyping 
    • DNA content and cell cycle
    • Apoptosis and necrosis
    • Cytotoxicity assays
    • Oxidative stress
    • Intracellular cytokine 
    • Signal transduction 
    • Cell activation
  • Cell sorting
    • Sterile cell sorting
    • Single cell sorting
  • Magnetic cell sorting
    • Positive cell isolation
    • Negative cell isolation
    • Rare cell enrichment
  • Immune monitoring
    • Peripheral blood cell isolation
    • Plasma/serum isloation
    • Cell banking services
    • Cytokine/growth factor analysis 
    • ELISA 
    • Elispot 
    • Luminex 
    • Leukocyte functional assays 
    • Proliferation 
    • Cytokine synthesis 
    • Antibody synthesis 
    • Cell cytotoxicity 
    • Cellular product services 
    • T cell expansion 
    • Macrophage/DC generation 
    • Antibody titers 
    • Immune Assay Design Services 
    • Research collaborations 

 

Requests for services can be made by contacting either the Flow Cytometry & Cell Sorting Lab or the Immune Monitoring Lab.

Reservations for services on particular FCIM instrumentation can be made through the PPMS website
 

Don Cohen, PhD
FCIM SRF Director

Jerry Woodward, PhD
Assistant Director of Immune Monitoring

Jamie Stugill, PhD
Assistant Director Flow Cytometry

Siva Gandhapudi, PhD
Manager of Immune Monitoring

Jennifer Strange Breeden
Manager of Flow Cytometry

Rita Avdiushko
Immune Monitoring Technician

Cuiping Zhang
Flow Cytometry Technician

 

Flow Cytometry and Cell Sorting Lab: 
Lab Suite 076, Healthy Kentucky Research Building 
Telephone: 859-323-6859 

Immune Monitoring Lab: 
Lab Suite 076A, Healthy Kentucky Research Building 
Telephone: 859-323-5584

Visit FCIM main website for facility brochure and more information.

Prices and lab policies for services can be found here

Where is the FCIM located?

The core is located in two adjoining labs, Lab Suite 076 (Flow Cytometry) and Lab Suite 076A (Immune Monitoring) in the Healthy Kentucky Research Building (HKRB).

What are the FCIM hours?

Staff are at the facility Monday through Friday, 9:30 am - 5:00 pm. 

Who should I contact regarding scheduling and general questions about services and billing for Flow Cytometry?

Please contact Jennifer Strange by email or by calling 859-323-6859.

Who should I contact regarding scheduling and general questions about services and billing for Immune Monitoring?
Please contact Siva Gandhapudi, PhD by email or at 859-323-5584

Who should I contact concerning experimental design, assay development, and biosafety issues?

Please contact facility director, Don Cohen, Ph.D., 859-323-5131, Jamie Sturgill, Ph.D. (assistant director), 859-323-7277, or Jerry Woodward, Ph.D. (associate director) at 859-323-5538.

quotation marks

Acknowledge the FCIM SRF

Investigators are required to acknowledge the Markey Cancer Center Flow Cytometry and Immune Monitoring Shared Resource Facility (FCIM SRF) in any publications that result from the use of biospecimens or information received through the MCC FCIM SRF. Please use the following statement to acknowledge the FCIM SRF.

“This research was supported by the Flow Cytometry and Immune Monitoring Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558).”

Markey Cancer Center is NCI-designated

The UK Markey Cancer Center was first designated by the National Cancer Institute in 2013 – a distinction that recognizes our extraordinary ability to provide world-class care for our patients. We are the only NCI-designated cancer center in Kentucky and one of only 71 in the nation.

LEARN MORE